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Please use this identifier to cite or link to this item: http://hdl.handle.net/10271/3215

Title: LUC7L3/CROP inhibits replication of hepatitis B virus via suppressing enhancer II/basal core promoter activity
Other Titles: LUC7L3/CROPはエンハンサーII/ベーサルコアプロモーターを抑制することによりB型肝炎ウイルスの複製を阻害する
Authors: Li, Yuan
李, 媛
Keywords: Hepatitis B virus
Issue Date: 18-Nov-2016
Publisher: Nature
Abstract: The core promoter of hepatitis B virus (HBV) genome is a critical region for transcriptional initiation of 3.5 kb, pregenome and precore RNAs and for the viral replication. Although a number of host-cell factors that potentially regulate the viral promoter activities have been identified, the molecular mechanisms of the viral gene expression, in particular, regulatory mechanisms of the transcriptional repression remain elusive. In this study, we identified LUC7 like 3 pre-mRNA splicing factor (LUC7L3, also known as hLuc7A or CROP) as a novel interacting partner of HBV enhancer II and basal core promoter (ENII/BCP), key elements within the core promoter, through the proteomic screening and found that LUC7L3 functions as a negative regulator of ENII/BCP. Gene silencing of LUC7L3 significantly increased expression of the viral genes and antigens as well as the activities of ENII/BCP and core promoter. In contrast, overexpression of LUC7L3 inhibited their activities and HBV replication. In addition, LUC7L3 possibly contributes to promotion of the splicing of 3.5 kb RNA, which may also be involved in negative regulation of the pregenome RNA level. This is the first to demonstrate the involvement of LUC7L3 in regulation of gene transcription and in viral replication.
Description: 浜松医科大学学位論文 医博第742号(平成29年2月9日)
URI: http://hdl.handle.net/10271/3215
DOI: info:doi/10.1038/srep36741
PubMed ID: info:pmid/27857158
Academic Degrees and number: 13802甲第742号
Degree-granting date: 2017-02-09
Degree name: 博士(医学)
Degree-granting institutions: 浜松医科大学
Appears in Collections:2010 博士(論文)

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