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Please use this identifier to cite or link to this item: http://hdl.handle.net/10271/3203

Title: Accumulation of arachidonic acid-containing phosphatidylinositol at the outer edge of colorectal cancer
Other Titles: 大腸がん辺縁にはアラキドン酸含有ホスファチジルイノシトールが集積する
Authors: Hiraide, Takanori
平出, 貴乗
Issue Date: 20-Jul-2016
Publisher: Nature Publishing Group
Abstract: Accumulating evidence indicates that cancer cells show specific alterations in phospholipid metabolism that contribute to tumour progression in several types of cancer, including colorectal cancer. Questions still remain as to what lipids characterize the outer edge of cancer tissues and whether those cancer outer edge-specific lipid compositions emerge autonomously in cancer cells. Cancer tissue-originated spheroids (CTOSs) that are composed of pure primary cancer cells have been developed. In this study, we aimed to seek out the cancer cell-autonomous acquisition of cancer outer edge-characterizing lipids in colorectal cancer by analysing phospholipids in CTOSs derived from colorectal cancer patients with matrix-assisted laser desorption/ionization (MALDI)-imaging mass spectrometry (IMS). A signal at m/z 885.5 in negative ion mode was detected specifically at the surface regions. The signal was identified as an arachidonic acid (AA)-containing phosphatidylinositol (PI), PI(18:0/20:4), by tandem mass spectrometry analysis. Quantitative analysis revealed that the amount of PI(18:0/20:4) in the surface region of CTOSs was two-fold higher than that in the medial region. Finally, PI(18:0/20:4) was enriched at the cancer cells/stromal interface in colorectal cancer patients. These data imply a possible importance of AA-containing PI for colorectal cancer progression, and suggest cells expressing AA-containing PI as potential targets for anti-cancer therapy.
Description: 浜松医科大学学位論文 医博第737号(平成28年9月16日)
URI: http://hdl.handle.net/10271/3203
DOI: info:doi/10.1038/srep29935
PubMed ID: info:pmid/27435310
Academic Degrees and number: 13802甲第737号
Degree-granting date: 2016-09-16
Degree name: 博士(医学)
Degree-granting institutions: 浜松医科大学
Appears in Collections:2010 博士(論文)

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