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Please use this identifier to cite or link to this item: http://hdl.handle.net/10271/3199

Title: UV damage-induced phosphorylation of HBO1 triggers CRL4DDB2-mediated degradation to regulate cell proliferation
Other Titles: 紫外線損傷により誘導されるHBO1のリン酸化はCRL4DDB2による分解のトリガーとなり細胞増殖を調整する
Authors: Matsunuma, Ryoichi
松沼, 亮一
Issue Date: Feb-2016
Publisher: American Society for Microbiology
Abstract: Histone acetyltransferase binding to ORC-1 (HBO1) is a critically important histone acetyltransferase for forming the prereplicative complex (pre-RC) at the replication origin. Pre-RC formation is completed by loading of the MCM2-7 heterohexameric complex, which functions as a helicase in DNA replication. HBO1 recruited to the replication origin by CDT1 acetylates histone H4 to relax the chromatin conformation and facilitates loading of the MCM complex onto replication origins. However, the acetylation status and mechanism of regulation of histone H3 at replication origins remain elusive. HBO1 positively regulates cell proliferation under normal cell growth conditions. Whether HBO1 regulates proliferation in response to DNA damage is poorly understood. In this study, we demonstrated that HBO1 was degraded after DNA damage to suppress cell proliferation. Ser50 and Ser53 of HBO1 were phosphorylated in an ATM/ATR DNA damage sensor-dependent manner after UV treatment. ATM/ATR-dependently phosphorylated HBO1 preferentially interacted with DDB2 and was ubiquitylated by CRL4DDB2. Replacement of endogenous HBO1 in Ser50/53Ala mutants maintained acetylation of histone H3K14 and impaired cell cycle regulation in response to UV irradiation. Our findings demonstrate that HBO1 is one of the targets in the DNA damage checkpoint. These results show that ubiquitin-dependent control of the HBO1 protein contributes to cell survival during UV irradiation.
Description: 浜松医科大学学位論文 医博第733号(平成28年7月22日)
URI: http://hdl.handle.net/10271/3199
DOI: info:doi/10.1128/MCB.00809-15
PubMed ID: info:pmid/26572825
Academic Degrees and number: 13802甲第733号
Degree-granting date: 2016-07-22
Degree name: 博士(医学)
Degree-granting institutions: 浜松医科大学
Appears in Collections:2010 博士(論文)

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