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Please use this identifier to cite or link to this item: http://hdl.handle.net/10271/2911

Title: MALDI-TOF mass spectrometric determination of 11 phenothiazines with heavy side chains in urine
Authors: Minakata, Kayoko
Yamagishi, Itaru
Nozawa, Hideki
Gonmori, Kunio
Hasegawa, Koutaro
Wurita, Amin
Suzuki, Masako
Watanabe, Kanako
Suzuki, Osamu
Keywords: MALDI-TOF mass spectrometry
α-Cyano-4-hydroxy cinnamic acid (CHCA)
Phenothiazines
Urine
Issue Date: Jan-2013
Publisher: Springer Japan
Abstract: A rapid screening method was developed for the determination of phenothiazines by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS). In this method, α-cyano-4-hydroxy cinnamic acid (CHCA) was used as the matrix to assist the ionization of phenothiazines. The identification of 11 phenothiazines with heavy side chains was performed by their protonated molecular ions [M + H]+ at m/z = 340 – 447, and the quantification was achieved using triflupromazine at m/z = 353 as the internal standard (IS). The relative ionization efficiencies of 11 phenothiazines and IS on MALDI-TOF-MS were different from those on ESI-TSQ-MS, but the product ion spectra on MALDI-MS-MS were quite similar to those on ESI-MS-MS except in the case of flupentixol. The limit of detection was 0.3 ng/ml with its quantification range of 1 – 50 ng/ml urine in the best case, and limit of detection was 5 ng/ml with its quantification range of 10 – 100 ng/ml urine in the worst case for 10 phenothiazines except thiethylperazine. Present method provides a simple and high throughput method for the screening of these phenothiazines using only 20 μl of urine. To our knowledge, this study is the first trial to analyze phenothiazines by MALDI-TOF MS (-MS).
URI: http://hdl.handle.net/10271/2911
DOI: info:doi/10.1007/s11419-012-0157-2
Appears in Collections:1010 雑誌掲載論文

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